Product Spotlights

Transfection of 1. DNA in vitro - jetPRIME™

 

Manufacturer: Polyplus-transfection

 

If you routinely perform transient gene expression studies and/or gene silencing by RNA interference, the new powerful jetPRIME™ is the reagent for you!

Transfection with this versatile reagent requires low quantities of nucleic acid and small amounts of reagent. Therefore, jetPRIME™ is both gentle to cells and economical. A simple and convenient protocol ensures that it’s easy to scale up or scale down.

Features/Benefits:

  • Outstanding DNA transfection efficiency
  • Economical: less DNA and less reagent needed
  • Better cell viability
  • Excellent gene silencing
  • Cotransfection of DNA & siRNA
  • shRNA and miRNA plasmid transfection
  • Convenient protocol

Specifications:

 

Catalog Number Description
114-01

jetPRIME kit 0.1 ml of jetPRIME + 5 ml of jetPRIME buffer

114-07

jetPRIME kit 0.75 ml of jetPRIME + 40 ml of jetPRIME buffer

114-15

jetPRIME kit 1.5 ml of jetPRIME + 2 x (40 ml of jetPRIME buffer)

114-75

jetPRIME kit 5 x (1.5 ml of jetPRIME) + 1 x 55 ml of jetPRIME buffer 10x (conc.)

114-75C

jetPRIME kit 5 x (1.5 ml of jetPRIME) + 1 x 120 ml of jetPRIME buffer 5x (conc.)

 

1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request

Contact us for a trial size of jetPRIME™ DNA & siRNA transfection reagent. Special offer with your first order of jetPRIME™!

For more information on jetPRIME™, click here.

 

 

 

Fetal Bovine serum

 

Manufacturer: Tico Europe

Tico Europe is a Dutch producer of Fetal Bovine Serum. As a leading supploer of an impressive range of cell culture products. Tico Europe provides absolute commitment to purity and trace-ability, first class service and timely delivery.

Finished Product Specifications

Test

Specifications

Endotoxin (Limulus Amebocyte Lysate – Gel Clot)

≤ 10 EU / ml

Hemoglobin (spectrophotometric)

≤ 20 mg / dl

Sterility Testing (current USP) for Bacteria and Fungi

Negative for growth

Virus Testing- Modified 9CFR

 

Bluetonque

Not Detected

Bovine Adenovirus

Not Detected

Bovine Parvovirus

Not Detected

Bovine Respiratory Syncytial Virus

Not Detected

Bovine Viral Diarrhea Virus

Not Detected

Rabies

Not Detected

Reovirus

Not Detected

Cytopathogenic Agents (IBR)

Not Detected

Hemadsorbing Agents (PI3)

Not Detected

Mycoplasma

 

Large Volume, Direct Culture

Not Detected

pH 6.8 - 8.2
Osmolarity 260 - 340
Identity Bovine

 

 

Fast Tissue-to-PCR Kit (#K1091) - Rapid extraction and amplification of DNA

 

Manufacturer: Fermentas

 

The Fast Tissue-to-PCR kit is a complete system for rapid extraction and amplification of genomic DNA from animal tissues.There is no need for procedures as tissue mechanical disruption, overnight digestion, phenol extraction, DNA precipitation or column purification.

The procedure involves short 10 minute incubation of the sample in the Tissue Lysis Solution supplemented with Proteinase K at room temperature. Then the Neutralization Solution T is added, and the tissue extract is ready for PCR with the Tissue Green PCR Master Mix (2X).

Feature / Benefits: 

  • Fast sample lysis during 10 min incubation at room temperature
  • Efficient PCR genotyping
  • Direct loading of PCR product on gel
  • Compatibility with various sample material: tissues from mice, fish and insects, human hair and saliva

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Components

  • Tissue Lysis Solution
  • Proteinase K
  • Neutralization Solution T
  • Tissue Green PCR Master Mix (2X)
  • Water, nuclease-free
  • Detailed Protocol

Figure 2. Multiplex PCR. 
Genomic DNA from mouse tail, ear clip and finger was extracted and amplified with Fast Tissue-to-PCR Kit. 114, 214 and 388 bp PCR products were generated using three sets of primer pairs.
M – GeneRuler™ Express DNA Ladder (#SM1551)
1, 2 – mouse ear clip
3, 4 – mouse tail
5, 6 – mouse finger

 

Epitope Tag Antibody – Detection of Tag-fusion proteins

 

Manufacturer: Nacalai Tesque

 

Nacalai Tesque carries an impressive variety of antibodies.Apart from a wide range of secondary antibodies, recently tag antibodies are developed in increased numbers. Currently available tagged antibody types are: GFP, c-Myc, His, V5, GST. Most of these types are available in POD coupled form as well. Uniquely, GFP (Rat IgG2a) is suitable for immunohistochemical detection!

Feature / Benefits: 

  • Immunohistochemical application
  • Rat monoclonal antibody

Specifications:

Product name

Grade

Storage

Code No.

Quantily

Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

BC

R

04404-26

50µg

04404-84

200µg

Anti-c-Myc (Mouse IgG1-κ), Monoclonal (MC045)

BC

R

04362-76

50µg

04362-34

200µg

Anti-GFP (Mouse IgG1-κ), Monoclonal (GF200)

BC

R

04363-66

50µg

04363-24

200µg

Anti-GST (Mouse IgG2a-κ), Monoclonal (GS019)

BC

R

04435-84

50µg

04435-26

200µg

Anti-V5 (Mouse IgG1-κ), Monoclonal (V5005)

BC

R

04434-94

50µg

04434-36

200µg

Anti-6xHis (Mouse IgG1-κ), Monoclonal (HI192)

BC

R

04428-26

50µg

04428-84

200µg

 

Signal Enhancer Hikari - Signal Enhancer for Western Blotting and ELISA

 

Manufacturer: Nacalai Tesque

 

Signal Enhancer HIKARI resolve the problems of low sensitivity and high background often encountered during procedures such as Western blotting and ELISA. Witness a more effective assay with Signal HIKARI instead of conventional diluents such as PBS and TBS to detect the protein of interest.

Features:

  • Enhances antigen-antibody reactions 
  • Reduces background 
  • Works with any substrate 
  • Ready-to-use reagent 

Ordering information

Product name Kit Contents Grade Storage Product No. PKG Size
Signal Enhancer HIKARI for Western Blotting and ELISA

Solution A for primary antibodies

Solution B for secondary antibodies

SP 4°C 02267-41 1set
(50ml each)
Signal Enhancer HIKARI for Western Blotting and ELISA Solution A 02272-74 250ml
Signal Enhancer HIKARI for Western Blotting and ELISA Solution B 02297-64 250ml
 
 
Detection enhancement of Rac1 with treatment of HIKARI in Western blotting
Treatment with Signal Enhancer HIKARI resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.

 

Signal Enhancer Hikari for Immunostain – Detect protein of interest with Immunostain procedures

 

Manufacturer: Nacalai Tesque

 

Signal Enhancer HIKARI for Immunostain resolves the problems of low sensitivity and high background often encountered during immunostain procedures. Signal Enhancer HIKARI produces a significant increase in the ability to detect protein of interest and eliminate unwanted background.

Feature / Benefits:

  • Enhances signals 
  • Reduces background
  • Ready-to-use reagent
  • Works with any system - applicable to colorimetric, chemiluminescent and fluorescent detection

Specifications: Product information

Product Name Product Number PKG Size
Signal Enhancer HIKARI for Immunostain Trial Set* 02363-71 1 set (5 ml each)
Signal Enhancer HIKARI for Immunostain Solution A 02373-54 20ml
Signal Enhancer HIKARI for Immunostain Solution B 02375-34 20ml

*Note: Signal Enhancer HIKARI for Immunostain Solution A and B exhibit different enhancing effects, depending on antigens and antibodies. These solutions can be used independently and a Trial set is available to help researchers select the suitable solution for their use.

Brain tissue section was stained with secondary antibody conjugated with AF555 (red) and counter stained with Hoechst dye (blue).

 

RNAGEM – Extraction of RNA from mammalian cell culture

 

Manufacturer: ZyGEM

 

RNAGem™ tissue RNA extraction kits feature a new formulation of enzymatic technologies that uses a single-tube process to provide complete extraction of RNA. This includes smaller RNA molecules such as noncoding RNA and microRNA that may be excluded by other RNA extraction methods. The RNAGem kits deliver high molecular weight RNA and good transcriptome profiles. They are suitable for complete gene-expression and noncoding RNA analyses.

Feature / Benefits:

  • Takes less than 10 minutes from start to finish, including handling time
  • Requires a single 5 minute incubation
  • Does not use GITC, phenol, chloroform or isopropanol
  • Delivers linear yields of total Nucleic Acid from 1 to 105 cells
  • Delivers linearity that is matched between high and low copy genes
  • Does not discriminate RNA molecules based on size

Kit Components of RNAGEM™ Tissue

  • RNAGEM enzyme
  • 10 x SILVER buffer
  • 10 x TE storage buffer
  • QuickStart Guide

 

Aviva Western Blot Reagent Kit

 

Manufacturer: Aviva Systems Biology

 

Aviva Systems Biology's new Western Blot Reagent Kits provide all the reagents required for conducting high quality, validated western blot analysis in one simple kit. Conditions and protocols have been optimized for easy visualization of each specific protein target.

Each kit includes: 

Primary Target Specific Antibody - WB Validated
Positive Control Lysate - Insures Antibody is Performing Properly 
Blocking Peptide - To Confirm Antibody Target Specificity
Secondary Antibody - Suitable for WB Analysis

Features/Benefits:

  • Includes all reagents required for running validated Western Blotting assays
  • Simple and validated protocols 
  • 84 kits available in a comprehensive range of key target areas

For more information please click here.

 

DNAgard™ Tissues & Cells - Stabilize DNA in tissue for at least 2 months at ambient temperature

 

Manufacturer: Biomatrica

 

 

DNAgard is a new Biomatrica product for the preservation and storage of DNA in tissues and cell culture lines at room temperature. DNAgard is an aqueous, non-toxic tissue and cell storage solution that permeates cells and stabilizes intracellular DNA in unfrozen samples. A unique dry-down feature allows extended storage time for one year at room temperature. Extraction by standard techniques yields high quality DNA ready for all downstream analyses.

 

Find out more on DNAgard™ Tissues & Cells and other products from Biomatrica here.

 

Restriction Enzymes

 

Manufacturer: Fermentas

 

FERMENTAS recently launched their new line of restriction enzymes called FastDigest®.

Fermentas FastDigest® enzymes is a premium line of restriction enzymes for easy digestion of DNA. It save time - Digest DNA in just 5 minutes!

All FastDigest® enzymes work in the same reaction buffer at the same temperature, therefore multiple digestions on DNA in any combination of FastDigest® enzymes are simple!

For more information, please call us.

Features/Benefits:

  • All enzymes work in same reaction conditions
  • No more sequential digestions
  • No more tedious buffer changes
  • No more concerns about star activity