PCR

Toyobo is a leading manufacturer of enzymes and reagents for life science research and industrial fields based on our original technologies about PCR enzymes. It consists of specially engineered ezymes with high efficiency according to various unique PCR enzymes, kits and PCR related products as shown below: 

Enzyme Product Name Application PCR Product Ends

 

High fidelity DNA polymerase

KOD -Plus- Neo    Cloning blunt
KOD FX Neo - Genotyping - Cloning high G/C

*applicable with crude sample

blunt
KOD Dash - Colony PCR - Detection

mixed (blunt &
3'-dA)

KOD -Plus- Mutagenesis Kit  
Tth DNA polymerase rTth DNA Polymerase  - General PCR  - RT-PCR 3'-dA
RT-PCR Quick Master Mix    One-step RT-PCR 3'-dA
Taq DNA polymerase Quick Taq® HS DyeMix  - Colony PCR  - General PCR 3'-dA
Blend Taq® -Plus-  - General PCR (longer target)  - Colony PCR  - Detection mixed
(blunt &
3'-dA)

cDNA synthesis Kits

Toyobo has various kits for cDNA synthesis based on the genetically improved M-MLV reverse transcriptase "ReverTra Ace®". ReverTra Ace -α-® is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®", as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR.

Product Name Application Long cDNA
synthesis
Master Mix Recommend PCR enzymes
ReverTra Ace -α-® Conventional
RT-PCR
++
(> 10 kb)
  KOD –Plus- series
KOD FX series
Taq-based PCR reagent
ReverTra Ace® qPCR RT Kit qPCR +
(< 5 kb)
  >THUNDERBIRD®series
Realtime PCR Master Mix series
KOD SYBR® qPCR Mix
KOD –Plus- series
KOD FX series
Taq-based PCR reagent
ReverTra Ace® qPCR RT Master Mix qPCR +
(< 5 kb)
ReverTra Ace® qPCR RT Master Mix with gDNA Remover qPCR +
(< 5 kb)
SuperPrep® Cell Lysis & RT Kit for qPCR qPCR from
cultured cells
+
(< 5 kb)