Stem Cell

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Brand Name Catalog Number Size
Revive Organtech CRYO-GOLD Cryopreservation Medium RO10003-01 100 mL
ReproCELL Dissociation Solution for Human ES/iPS Cells RCHETP002 30 mL
CryoStem™ Freezing Medium 01-0013-50 50 ml
ReproCELL Freezing Medium for Human ES/iPS Cells RCHEFM001 25 mL
Stemgent® hES Cell Cloning & Recovery Supplement 01-0014-500 5 x 100 μl
Stemgent® MesoFate™ Cardiomyocyte Differentiation Medium 00-0072 500 ml + supplement
ReproCELL Primate ES Cell Culture Medium RCHEMD001 500 mL
ReproCELL ReproCoat Coating Solution RCHEOT001 500 mL
ReproCELL ReproCryo DMSO Free Cryopreservation Medium RCHEFM002 50 mL
ReproCELL ReproFF2 Cell Culture Medium RCHEMD006 500ML
Pluriton™ Reprogramming Medium 00-0070 500 ml
ReproCELL ReproStem Cell Culture Medium RCHEMD005 500 mL
ReproCELL ReproXF Culture Medium RCHEMD007 500 mL
Ajinomoto StemFit® Basic02 Cell Culture Medium ASB01 500 mL
NutriStem™ XF/FF Culture Medium for Human iPS and ES Cells 01-0005 500 ml
01-0005-100 100 ml

 

Cytokines / Proteins

Cytokines (growth factors) play a central role in the self-renewal and differentiation of embryonic stem (ES) induced pluripotent (iPS) cells, as well as, in the reprogramming of somatic cells to iPS cells.

Cytokines typically serve as messengers between cells and elicit a cascade of intracellular signaling sequences by binding to transmembrane receptors.
The timing, localization, concentration, and the combination of various factors is crucial to cell type specification and maturation, as well as, establishing
the essential environment for the maintenance of cells in vivo and in vitro.

View product sheet.

Stemgent® Stemfactor™ recombinant human proteins and cytokines are extensively tested to ensure purity, stability, and high biological activity.

Brand Name Catalog Number Size
Stemfactor™ Activin A, Human Recombinant 03-0001 5 μg
Stemfactor™ B18R Recombinant Protein, Carrier-free 03-0017 50 ug
Stemfactor™ BMP-4, Human Recombinant 03-0007 10 μg
Stemfactor™ FGF-basic, Human Recombinant 03-0002 50 μg
Stemfactor™ LIF, Human Recombinant 03-0016 1 ml, 10 μg/ml
03-0016-100 1 ml, 100 μg/ml
Stemfactor™ LIF, Mouse Recombinant 03-0011 1 ml, 10 μg/ml
03-0011-100 1 ml, 100 μg/ml
Stemfactor™ Noggin, Human Recombinant 03-0006 10 μg
Stemfactor™ Sonic Hedgehog, Human Recombinant 03-0008 25 μg
Stemfactor™ TGF-β1, Human Recombinant 03-0004 5 μg

 

Reprogramming Systems

RNA Reprogramming Systems for Fibroblasts and Blood

Highly Efficient Reprogramming

The Stemgent RNA Reprogramming System provides efficiencies greater than 1% as compared to other methods, which yield reprogramming efficiencies varying from 0.00001% to 0.1%. In addition to increased yield of colonies and fast reprogramming kinetics, RNA-based reprogramming does not require laborious multi-step downstream passaging or screening for viral vector retention or genomic integration.

Fibroblast Reprogramming

The Stemgent microRNA-boosted mRNA reprogramming system, combining the mRNA Reprogramming Kit (00-0071)  with the microRNA Booster Kit (00-0073) and the Stemfect™ RNA Transfection Kit (00-0069), provides high-quality reprogramming of adult and neonatal fibroblasts. The microRNA Booster Kit (00-0073) increases the success with highly refractory target cells, improves efficiency, and reduces the cost per well.

Blood Reprogramming

The Stemgent StemRNA™-SR Reprogramming Kit (00-0075), using self-replicative RNA, is the first RNA-based reprogramming kit for blood derived cells.  Starting with genetically stable endothelial progenitor cells (EPCs), easily isolated from whole blood or frozen MNCs, this technology can efficiently generate iPSCs that are free integrated vector and ready to differentiate or scale up.

 

Brand Name Catalog Number Size
Stemgent® c-Myc mRNA, Human 05-0018 20 μg
Stemgent® eGFP mRNA 05-0020 20 μg
Stemgent® Klf4 mRNA, Human 05-0015 20 μg
Stemgent® L-Myc mRNA, Human 05-0022 20 μg
Stemgent® Lin28 mRNA, Human 05-0017 20 μg
Stemgent® microRNA Booster Kit 00-0073 1 kit
Stemgent® mRNA Reprogramming Factors Set: hOKSML 00-0067 1 Set
Stemgent® mRNA Reprogramming Kit 00-0071 1 kit
Stemgent® Nanog mRNA, Human 05-0021 20 μg
Stemgent® nGFP mRNA 05-0019 20 μg
Stemgent® Oct4 mRNA, Human 05-0014 20 μg
Stemgent® Sox2 mRNA, Human 05-0016 20 μg
Stemgent® StemRNA™-NM Reprogramming Kit 00-0076 1 kit
Stemgent® StemRNA™-SR Reprogramming Kit 00-0075 1 Kit

 

Small Molecules for Stem Cell Research

Stemgent® offers a unique collection of small molecules, Stemolecules™, that can be used to dissect and modulate key signaling pathways involved in the self-renewal and differentiation of pluripotent stem cells, cellular reprogramming for the generation of induced pluripotent stem (iPS) cells, and induction and maintenance of the naïve state.

Stemolecules are stringently tested for high purity (≥98%), structurally verified by NMR and mass spectrometry, and tested for cytotoxicity on stem cells. 
Many Stemolecules are used routinely at ReproCELL to enhance iPS cell generation and maintain pluripotent stem cell cultures.

Brand Name Catalog Number Size
Stemolecule™ A769662 04-0052 10 mg
Stemolecule™ A83-01 04-0014 2 mg
04-0014-10 10 mg
Stemolecule™ ALK5 Inhibitor (RepSox) 04-0015 1 mg
Stemolecule™ All-Trans Retinoic Acid 04-0021 100 mg
Stemolecule™ AM580 04-0077 5 mg
Stemolecule™ BI-D1870 04-0051 10 mg
Stemolecule™ BIO 04-0003 2 mg
Stemolecule™ BIX01294 04-0002 5 mg
Stemolecule™ CD437 04-0078 5 mg
Stemolecule™ CHIR99021 04-0004 2 mg
04-0004-10 10 mg
Stemolecule™ CHIR99021 in Solution 04-0004-02 2 mg (10 mM)
Stemolecule™ Cyclopamine 04-0022 2 mg
Stemolecule™ DAPT 04-0041 5 mg
Stemolecule™ Dorsomorphin 04-0024 2 mg
Stemolecule™ Doxycycline hyclate 04-0016 10 mg
Stemolecule™ ec23 SRP002 5mg
SRP002-2 2 x 5mg
Stemolecule™ Forskolin 04-0025 10 mg
Stemolecule™ GDC-0941 04-0047 5 mg
Stemolecule™ GSK429286A 04-0049 2 mg
Stemolecule™ IDE-1 04-0026 2 mg

 

Brand Name Catalog Number Size
Stemolecule™ IDE-2 04-0027 2 mg
Stemolecule™ IM-12 04-0081 2 mg
Stemolecule™ IPA-3 04-0037 5 mg
Stemolecule™ KAAD-Cyclopamine 04-0028 100 μg
Stemolecule™ LDN-193189 04-0074 2 mg
04-0074-10 10 mg
Stemolecule™ LDN-193189 in Solution 04-0074-02 2 mg (10 mM)
Stemolecule™ LY411575 04-0054 5 mg
Stemolecule™ PD0325901 04-0006 2 mg
04-0006-10 10 mg
Stemolecule™ PD0325901 in Solution 04-0006-02 2 mg (10 mM)
Stemolecule™ PD173074 04-0008 2 mg
Stemolecule™ Pifithrin-μ 04-0039 5 mg
Stemolecule™ Purmorphamine 04-0009 5 mg
Stemolecule™ Pyrintegrin 04-0072 1 mg
Stemolecule™ R(+)Bay K 8644 04-0013 5 mg
Stemolecule™ RG108 04-0001 10 mg
Stemolecule™ ROCK II Inhibitor 04-0030 2 mg
Stemolecule™ SB431542 04-0010 5 mg
04-0010-10 10 mg
Stemolecule™ SB431542 in Solution 04-0010-05 5 mg (10 mM)
Stemolecule™ SB590885 04-0080 2 mg
Stemolecule™ SC1 (Pluripotin) 04-0011 1 mg

 

Brand Name Catalog Number Size
Stemolecule™ SHH Antagonist 04-0032 2 mg
Stemolecule™ SMO Antagonist 04-0031 2 mg
Stemolecule™ Sodium Butyrate 04-0005 500 mg
Stemolecule™ Thiazovivin 04-0017 1 mg
Stemolecule™ Valproic Acid 04-0007 5 g
Stemolecule™ WH-4-023 04-0079 2 mg
Stemolecule™ Wnt Inhibitor IWP-2 04-0034 2 mg
Stemolecule™ Wnt Inhibitor IWP-3 04-0035 2 mg
Stemolecule™ Wnt Inhibitor IWP-4 04-0036 2 mg
Stemolecule™ XAV939 04-0046 2 mg
Stemolecule™ Y27632 04-0012 2 mg
04-0012-10 10 mg
Stemolecule™ Y27632 Dihydrochloride Hydrate 04-0012-H-10 10 mg
Stemolecule™ Y27632 in Solution 04-0012-02 2 mg (10 mM)