Cell Culture

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As one of the leading life science reagent suppliers in Japan, Nacalai Tesque is also Japan's homegrown brand that offers high quality cell culture reagents for researchers. Its culture reagents have been published in a lot publications that includes Nature an international leading scientific journal.

Culture Media

  • Animal origin-free
  • Sterility tested for bacteria, fungus and mycoplasma
  • Endotoxin tested
  • pH 7.1-7.5
Product name Product No. PKG Size
DMEM (1.0g/l Glucose) with L-Gln and Sodium Pyruvate, liquid 08456-65 500 ml
08456-36 10 bottles x 500 ml
DMEM (1.0g/l Glucose) with Sodium Pyruvate, without L-Gln and Phenol Red, liquid 08490-05 500 ml
DMEM (4.5g/l Glucose) with L-Gln and HEPES, without Sodium Pyruvate, liquid 08457-55 500 ml
DMEM (4.5g/l Glucose) with L-Gln and Sodium Pyruvate, liquid 08458-45 500 ml
08458-16 10 bottles x 500 ml
DMEM (4.5g/l Glucose) with L-Gln, without Sodium Pyruvate, liquid 08459-35 500 ml
08459-64 10 bottles x 500 ml
DMEM (4.5g/l Glucose) without L-Gln, Sodium Pyruvate and Phenol Red, liquid 08489-45 500 ml
DMEM (4.5g/l Glucose) without L-Gln and Sodium Pyruvate, liquid 08488-55 500 ml
DMEM (4.5g/l Glucose) with Sodium Pyruvate, without L-Gln, liquid 11584-85 500 ml
DMEM (4.5g/l Glucose) with HEPES, without L-Gln and Sodium Pyruvate, liquid 11585-75 500 ml
DMEM (No Glucose) with L-Gln, without Sodium Pyruvate, liquid 09891-25 500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, liquid 08460-95 500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, without Phenol Red, liquid 05177-15 500 ml
DMEM/Ham's F-12 with L-Gln and Sodium Pyruvate, without HEPES, liquid 11581-15 500 ml
DMEM/Ham's F-12 with L-Gln and Sodium Pyruvate, without HEPES and Phenol Red, liquid 11582-05 500 ml
DMEM/Ham's F-12 with Sodium Pyruvate and HEPES, without L-Gln, liquid 11583-95 500 ml
DMEM/Ham's F-12 (No Glucose) with L-Gln and Sodium Pyruvate, liquid 09893-05 500 ml
IMDM with L-Gln and HEPES, liquid (Iscove's Modified Dulbecco's Medium) 11506-05 500 ml
Ham's F-12 with L-Gln, liquid 17458-65 500 ml
MEM with Earle's Salts and L-Gln, liquid 21442-25 500 ml
MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid 21443-15 500 ml
MEM (No Glucose) with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid 09848-05 500 ml
α-MEM with L-Gln, Ribonucleosides and Deoxyribonucleosides, liquid 21444-05 500 ml
α-MEM with L-Gln, without Ribonucleosides and Deoxyribonucleosides, liquid 21445-95 500 ml
RPMI 1640 with L-Gln and HEPES, liquid 30263-95 500 ml
RPMI 1640 with L-Gln, liquid 30264-85 500 ml
30264-56 10 bottles x 500 ml
RPMI 1640 with L-Gln, without Phenol Red, liquid 06261-65 500 ml
RPMI 1640 with L-Gln, liquid 05176-25 500 ml
RPMI 1640 (No Glucose) with L-Gln, liquid 09892-15 500 ml
 

 

Balanced Salines

Sterilized by 0.2 µm membrane filter, tested for bacteria, fungus, mycoplasma and endotoxin

Product name Product No. PKG Size
D-PBS(+) Preparation Reagent (Ca,Mg Solution) (100x) 02492-94 30 ml
D-PBS(-) without Ca and Mg, liquid  14249-95 500 ml
14249-24  10 bottles x 500 ml 
D-PBS(-) without Ca and Mg, liquid(10x) 11482-15 500 ml 
D-PBS(-) without Ca and Mg, Powder 07269-84 100 g
HBSS(+) with Ca, Mg and Phenol Red, liquid 17459-55 500 ml
HBSS(+) with Ca, Mg, without Phenol Red, liquid 09735-75 500 ml
HBSS(-) without Ca and Mg, with Phenol Red, liquid 17460-15 500 ml
HBSS(-) without Ca, Mg and Phenol Red, liquid 17461-05 500 ml

 

Supplements

Sterilized by 0.2 µm membrane filter, tested for bacteria, fungus, mycoplasma and endotoxin

Product name Product No. PKG Size
L-Alanyl-L-glutamine 01102-82 25 g
200mmol/l L‐Alanyl‐L‐glutamine Solution(100x) 04260-64 100 ml
200mM‐L‐Glutamine Stock Solution 16948-04 100 ml
1mol/l-HEPES Buffer Solution 17557-94 100 ml
MEM Non-Essential Amino Acids Solution(100x) 06344-14 20 ml
06344-56 100 ml
100mM-Sodium Pyruvate Solution(100x) 06977-34 100 ml
apo-Transferrin from Human 34401-84 100 mg
34401-55 500 mg
 

 

Cell Dissociation Reagents

Product name Product No. PKG Size
2.5g/l-Trypsin Solution 35555-54 100 ml
5.0g/l-Trypsin/5.3mmol/l-EDTA Solution 35556-44 100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution 35554-64 100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution, 
with Phenol Red
32777-44 100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution 35553-74 100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution, 
with Phenol Red
32778-34 100 ml
0.2g/l-EDTA Solution 14367-74 100 ml
 

 

Antibiotics

  • Animal origin-free
  • Sterility tested for bacteria, fungus and mycoplasma
  • Endotoxin tested
  • pH 7.1-7.5

 

Product name Application Product No. PKG Size
Actinomycin D Solution(1mg/ml) Other Antibiotics 00393-41 1 ml
Antibiotic-Antimycotic Mixed 
Stock Solution(100x)
Bacteria, Fungal, Yeast 02892-54 100 ml
Antibiotic-Antimycotic Mixed 
Stock Solution(100x)(Stabilized)
Bacteria, Fungal, Yeast 09366-44 100 ml
Colcemid Solution(10µg/ml) Other Antibiotics 09356-74 10 ml
G 418 Disulfate Selection Antibiotics 16512-36 250 mg
16512-81 1 g
16512-94 5 g
16512-52 25 g
G 418 Disulfate Other Antibiotics 08973-01 1 g
08973-14 5 g
G 418 Disulfate Aqueous Solution Selection Antibiotics 09380-86 20 ml
09380-44 100 ml
Gentamicin Sulfate Bacteria/Mycoplasma 08975-81 1 g
08975-94 5 g
Gentamicin Sulfate Solution (10mg/ml) Bacteria/Mycoplasma 16672-04 10 ml
Hygromycin B  Selection Antibiotics  07296-66 100 mg
07296-11 1 g
07296-24   5 g
Hygromycin B Solution Selection Antibiotics 09287-84 20 ml
Kanamycin Monosulfate Selection Antibiotics 08976-71 1 g
08976-84 5 g
Mitomycin C Solution(1mg/ml) Other Antibiotics 20898-21 1 ml
Penicillin-Streptomycin Mixed Solution
Penicillin 10,000u/ml, Streptomycin 10,000µg/ml
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
26253-84 100 ml
Penicillin-Streptomycin-Glutamine Mixed Solution
Penicillin 10,000u/ml, Streptomycin 10,000µg/ml, L-Glutamine 29.2mg/ml,
Sodium Chloride 0.14%, Citrate Buffer Solution 10mM
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
06168-34 100 ml
Penicillin-Streptomycin Mixed Solution (Stabilized)
Penicillin 10,000u/ml, Streptomycin 10,000µg/ml
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
09367-34 100 ml
Penicillin-Streptomycin Mixed Solution
Penicillin 5,000u/ml, Streptomycin 5,000µg/ml
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
26252-94 100 ml
Streptomycin Sulfate Gram-negative bacteria 32204-34 5 g
32204-92 25 g